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HPLC with Post-Column Derivatization with Alizarin for Determination of OATD-02, an Anticancer Arginase Inhibitor in Clinical Development

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dc.abstract.enThe aim of this study was to develop an analytical method for selective determination of OATD-02 by high-performance liquid chromatography (HPLC) with post-column derivatization and fluorescence detection (FLD). OATD-02, a new boronic acid derivative, is a highly potent anticancer arginase inhibitor in clinical development. Chromatographic analysis of OATD-02 poses problems because this molecule has weak ultraviolet absorption. The derivatization reaction was based on the reaction between boronic acid from OATD-02 and alizarin solution. The optimized mobile phase consisted of a mixture of sodium bicarbonate in water and acetonitrile at a flow rate of 0.50 mL/min. Alizarin solution in methanol was delivered at a flow rate of 0.50 mL/min. The fluorescent complexes were detected by a fluorescence detector (excitation and emission wavelengths at 470 and 580 nm, respectively). The present method demonstrated proper values for selectivity, linearity, recovery (>99%), precision (RSD: 0.6%), sensitivity (LOD: 20 µg/mL and LOQ: 50 µg/mL), stability of solutions, and robustness.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorBiesaga, Magdalena
dc.contributor.authorBlaszczyk, Roman
dc.contributor.authorTyszkiewicz, Magdalena
dc.contributor.authorSobolewska, Elzbieta
dc.date.accessioned2024-01-25T03:30:40Z
dc.date.available2024-01-25T03:30:40Z
dc.date.copyright2023-08-13
dc.date.issued2023
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number16
dc.description.versionFINAL_PUBLISHED
dc.description.volume13
dc.identifier.doi10.3390/APP13169201
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/108717
dc.identifier.weblinkhttps://www.mdpi.com/2076-3417/13/16/9201/pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofApplied Sciences (Switzerland)
dc.relation.pages9201
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enpost-column derivatization
dc.subject.enactive pharmaceutical ingredient
dc.subject.enarginase inhibitor
dc.subject.enHPLC
dc.titleHPLC with Post-Column Derivatization with Alizarin for Determination of OATD-02, an Anticancer Arginase Inhibitor in Clinical Development
dc.typeJournalArticle
dspace.entity.typePublication