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Smartphone-Assisted Protein to Creatinine Ratio Determination on a Single Paper-Based Analytical Device

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dc.abstract.enProteinuria is a condition in which an excessive amount of protein is excreted in urine. It is, among others, an indicator of kidney disease or risk of cardiovascular disease. Rapid and reliable diagnosis and monitoring of proteinuria is of great importance for both patients and their physicians. For that reason, a paper-based sensor for proteinuria diagnosis was designed, optimized, and validated utilizing smartphone-assisted signal acquisition. In the first step, a few commonly employed protein assays were optimized and compared in terms of analytical performance on paper matrix. The tetrabromophenol blue method was selected as the one providing a sufficiently low limit of detection (39 mg·L−1) on the one hand and appropriate long-term stability (up to 3 months) on the other hand. The optimized assay was employed for protein-to-creatinine ratio (PCR) determination on a single paper-based sensor. For both analytes the linear ranges were within the clinically relevant range. The analytical usefulness of the developed sensors was demonstrated by a PCR recovery study in artificial urine. The obtained PCR recoveries were from ca. 80 to 150%.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorKurdziałek, Karolina
dc.contributor.authorLewińska, Izabela
dc.contributor.authorTymecki, Łukasz
dc.date.accessioned2024-01-26T07:49:36Z
dc.date.available2024-01-26T07:49:36Z
dc.date.copyright2021-10-17
dc.date.issued2021
dc.description.accesstimeAT_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.number20
dc.description.versionFINAL_PUBLISHED
dc.description.volume26
dc.identifier.doi10.3390/MOLECULES26206282
dc.identifier.issn1420-3049
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/120035
dc.identifier.weblinkhttps://www.mdpi.com/1420-3049/26/20/6282/pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofMolecules
dc.relation.pages6282
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enprotein
dc.subject.encreatinine
dc.subject.enpaper-based analytical devices
dc.subject.ensmartphone
dc.subject.enalbumin to creatinine ratio
dc.subject.enproteinuria
dc.subject.entetrabromophenol blue
dc.subject.en3,5-dinitrobenzoic acid
dc.titleSmartphone-Assisted Protein to Creatinine Ratio Determination on a Single Paper-Based Analytical Device
dc.typeJournalArticle
dspace.entity.typePublication