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An Unprecedented Number of Cytochrome P450s Are Involved in Secondary Metabolism in Salinispora Species

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cris.lastimport.scopus2024-02-12T20:51:23Z
dc.abstract.enCytochrome P450 monooxygenases (CYPs/P450s) are heme thiolate proteins present in species across the biological kingdoms. By virtue of their broad substrate promiscuity and regio- and stereo-selectivity, these enzymes enhance or attribute diversity to secondary metabolites. Actinomycetes species are well-known producers of secondary metabolites, especially Salinispora species. Despite the importance of P450s, a comprehensive comparative analysis of P450s and their role in secondary metabolism in Salinispora species is not reported. We therefore analyzed P450s in 126 strains from three different species Salinispora arenicola, S. pacifica, and S. tropica. The study revealed the presence of 2643 P450s that can be grouped into 45 families and 103 subfamilies. CYP107 and CYP125 families are conserved, and CYP105 and CYP107 families are bloomed (a P450 family with many members) across Salinispora species. Analysis of P450s that are part of secondary metabolite biosynthetic gene clusters (smBGCs) revealed Salinispora species have an unprecedented number of P450s (1236 P450s-47%) part of smBGCs compared to other bacterial species belonging to the genera Streptomyces (23%) and Mycobacterium (11%), phyla Cyanobacteria (8%) and Firmicutes (18%) and the classes Alphaproteobacteria (2%) and Gammaproteobacteria (18%). A peculiar characteristic of up to six P450s in smBGCs was observed in Salinispora species. Future characterization Salinispora species P450s and their smBGCs have the potential for discovering novel secondary metabolites.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorSyed, Khajamohiddin
dc.contributor.authorNelson, David R.
dc.contributor.authorGront, Dominik
dc.contributor.authorKarpoormath, Rajshekhar
dc.contributor.authorSyed, Puleng Rosinah
dc.contributor.authorPadayachee, Tiara
dc.contributor.authorNzuza, Nomfundo
dc.contributor.authorMalinga, Nsikelelo Allison
dc.date.accessioned2024-01-24T16:43:51Z
dc.date.available2024-01-24T16:43:51Z
dc.date.copyright2022-04-21
dc.date.issued2022
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number5
dc.description.versionFINAL_PUBLISHED
dc.description.volume10
dc.identifier.doi10.3390/MICROORGANISMS10050871
dc.identifier.issn2076-2607
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/100952
dc.identifier.weblinkhttps://www.mdpi.com/2076-2607/10/5/871/pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofMicroorganisms
dc.relation.pages871
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.ennatural products
dc.subject.ensecondary metabolites
dc.subject.enactinomycete
dc.subject.enmarine
dc.subject.enSalinispora arenicola
dc.subject.encytochrome P450
dc.subject.enbiosynthetic gene clusters
dc.subject.engenome-data mining
dc.subject.endiversity
dc.subject.enStreptomyces
dc.subject.enMycobacterium
dc.titleAn Unprecedented Number of Cytochrome P450s Are Involved in Secondary Metabolism in Salinispora Species
dc.typeJournalArticle
dspace.entity.typePublication