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New, fast and cheap prediction tests for BRCA1 gene mutations identification in clinical samples.

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cris.lastimport.scopus2024-02-12T20:53:04Z
dc.abstract.enDespite significant progress in cancer therapy, cancer is still the second cause of mortality in the world. The necessity to make quick therapeutic decisions forces the development of procedures allowing to obtain a reliable result in a quick and unambiguous manner. Currently, detecting predictive mutations, including BRCA1, is the basis for effectively treating advanced breast cancer. Here, we present new\ninsight on gene mutation detection. We propose a cheap BRCA1 mutation detection tests based on the surface plasmon resonance (SPR) or quartz crystal microbalance with energy dissipation (QCM-D) response changes recorded during a hybridization process of an oligonucleotide molecular probe with DNA fragments, with and without the BRCA1 mutation. The changes in the morphology of the formed DNA layer caused by the presence of the mutation were confirmed by atomic force microscopy. The unique property of the developed SPR and QCM tests is really short time of analysis: ca. 6 min for\nSPR and ca. 25 min for QCM. The proposed tests have been verified on 22 different DNA extracted from blood leukocytes collected from cancer patients: 17 samples from patients with various BRCA1 gene mutation variants including deletion, insertion and missense single-nucleotide and 5 samples from patients without any BRCA1 mutation. Our test is a response to the need of medical diagnostics\nfor a quick, unambiguous test to identify mutations of the BRCA1 gene, including missense singlenucleotide\n(SNPs).
dc.affiliationUniwersytet Warszawski
dc.contributor.authorNowicka, Anna
dc.contributor.authorKudelski, Andrzej
dc.contributor.authorKowalik, Artur
dc.contributor.authorZięba, Sebastian
dc.contributor.authorSęk, Sławomir
dc.contributor.authorKowalczyk, Agata
dc.contributor.authorPotęga, Agnieszka
dc.contributor.authorGajda-Walczak, Aleksandra
dc.date.accessioned2024-01-25T13:47:49Z
dc.date.available2024-01-25T13:47:49Z
dc.date.copyright2023-05-05
dc.date.issued2023
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number1
dc.description.versionFINAL_PUBLISHED
dc.description.volume13
dc.identifier.doi10.1038/S41598-023-34588-9
dc.identifier.issn2045-2322
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/113569
dc.identifier.weblinkhttps://www.nature.com/articles/s41598-023-34588-9.pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofScientific Reports
dc.relation.pagesart.no. 7316
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enBRCA1 GENE MUTATIONS
dc.subject.enBREAST CANCER
dc.subject.enELECTROCHEMICAL DNA BIOSENSOR
dc.subject.enQUARTZ CRYSTAL MICROBALANCE-DISSIPATION
dc.subject.enSURFACE PLASMON RESONANCE
dc.titleNew, fast and cheap prediction tests for BRCA1 gene mutations identification in clinical samples.
dc.typeJournalArticle
dspace.entity.typePublication