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Prosecretory effect of loperamide in ileal and colonic mucosae of mice displaying high or low swim stress-induced analgesia associated with high and low endogenous opioid system activity

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dc.abstract.enBackground: Irritable bowel syndrome (IBS) is characterized by abdominal pain, bloating, and changes in bowel habit. The aim of this study was to characterize the effect of loperamide hydrochloride (LOP) and naloxone hydrochloride (NLX), an opioid agonist and antagonist, respectively, on electrolyte equilibrium in ileal and colonic mucosae and to estimate the possible influence of divergent activity of the endogenous opioid system (EOS) on IBS therapy. Methods: Two mouse lines bidirectionally selected for high (HA) and low (LA) swim stress-induced analgesia associated with high and low EOS activity were used in this study. To assess the effect of LOP and NLX on HA/LA lines in vivo, we used the castor oil-induced diarrhea model. Changes in electrolyte equilibrium were determined on the basis of short-circuit current (ΔIsc) in isolated mouse ileum and colon exposed to LOP and NLX and stimulated by forskolin (FSK), veratridine (VER), and bethanechol (BET). Key Results: In vivo, we found that LOP significantly prolonged time to appearance of diarrhea in HA and LA lines. In vitro, LOP and NLX increased ΔIsc in FSK- and VER-stimulated colonic tissue, respectively, in HA line. In the ileum, LOP increased ΔIsc in FSK- and VER-stimulated tissue and decreased ΔIsc in BET-stimulated tissues in HA line. Conclusions & Inferences: Individual differences in EOS activity may play a crucial role in the response to the IBS-D therapy, thus some patients may be at an increased risk of side effects such as constipation or diarrhea.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorMisicka-Kęsik, Aleksandra
dc.contributor.authorSacharczuk, Mariusz
dc.contributor.authorWasilewski, A
dc.contributor.authorFichna, Jakub
dc.date.accessioned2024-01-25T18:13:45Z
dc.date.available2024-01-25T18:13:45Z
dc.date.issued2018
dc.description.financeNie dotyczy
dc.description.number2
dc.description.volume30
dc.identifier.doi10.1111/NMO.13166
dc.identifier.issn1350-1925
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/117266
dc.identifier.weblinkhttp://onlinelibrary.wiley.com/
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofNeurogastroenterology and Motility
dc.relation.pagese13166
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enendogenous opioid system
dc.subject.enion transport
dc.subject.enirritable bowel syndrome
dc.subject.ensecretory diarrhea
dc.titleProsecretory effect of loperamide in ileal and colonic mucosae of mice displaying high or low swim stress-induced analgesia associated with high and low endogenous opioid system activity
dc.typeJournalArticle
dspace.entity.typePublication