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New strategy for the gene mutation identification using surface enhanced Raman spectroscopy (SERS)

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dc.abstract.enAn early and accurate diagnosis of a specific DNA mutations has a decisive role for effective treatment. Especially, when an immediate decision on treatment most needs to be made, the rapid and precise confirmation of clinical findings is vital. Herein, we show a new strategy for the gene mutation (BRAF c.1799T>A; p. V600E) identification using highly SERS-active and reproducible SERS substrate (photo-etched GaN covered with a thin layer of sputtered gold) and surface enhanced Raman scattering (SERS) spectroscopy. The detection is based on the conformation change (gauche → trans) of the alkanethiol linker modifying the capture DNA during the hybridization process. The value of the intensity ratio of the ν(C–S) bands of the trans and gauche conformer higher than 1.0 indicated the presence of mutation. The demonstrated new DNA SERS (bio)sensor is characterized by the low detection limit at the level of pg μL–1, wide analytical range from 6.75 pg μL–1 to 67.5 ng μL–1 and high selectivity. The proposed bioactive platforms, based on nanostructured GaN substrates modified with thiolated ssDNA (single stranded DNA) can be successfully used in the analysis of clinical samples.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorChłopek, Małgorzata
dc.contributor.authorKudelski, Andrzej
dc.contributor.authorNowicka, Anna
dc.contributor.authorDzięcielewski, Igor
dc.contributor.authorKowalik, Artur
dc.contributor.authorKrajczewski, Jan
dc.contributor.authorWeyher, Jan
dc.contributor.authorGóźdź, Stanisław
dc.contributor.authorKowalczyk, Agata
dc.date.accessioned2024-01-25T13:48:54Z
dc.date.available2024-01-25T13:48:54Z
dc.date.issued2019
dc.description.financeNie dotyczy
dc.description.volume132
dc.identifier.doi10.1016/J.BIOS.2019.03.019
dc.identifier.issn0956-5663
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/113627
dc.identifier.weblinkhttps://reader.elsevier.com/reader/sd/pii/S0956566319302222?token=797A3F516A1E0BB13810AA28258A7E697B67E271A2A5FC049E35C8D9F138D5C1B84A1B49EBB483420EE0FDED1E55106D
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofBiosensors and Bioelectronics
dc.relation.pages326-332
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enConformation analysis
dc.subject.enDNA recognition
dc.subject.enGene mutation identification
dc.subject.enSERS
dc.subject.enSurface analysis
dc.titleNew strategy for the gene mutation identification using surface enhanced Raman spectroscopy (SERS)
dc.typeJournalArticle
dspace.entity.typePublication