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Lipid membranes exposed to dispersions of phytantriol and monoolein cubosomes: Langmuir monolayer and HeLa cell membrane studies

cris.lastimport.scopus2024-02-12T20:53:38Z
dc.abstract.enThe interactions of liquid-crystalline nanoparticles based on lipid-like surfactants, glyceryl monooleate, monoolein (GMO) and 1,2,3-trihydroxy-3,7,11,15-tetramethylhexadecane, phytantriol (PT) with selected model lipid membranes prepared by Langmuir technique were compared. Monolayers of DPPC, DMPS and their mixture DPPC:DMPS 87:13 mol% were used as simple models of one leaflet of a cell membrane. The incorporation of cubosomes into the lipid layers spread at the air-water interface was followed by surface-pressure measurements and Brewster angle microscopy. The cubosome - membrane interactions lead to the fluidization of the model membranes but this effect depended on the composition of the model membrane and on the type of cubosomes. The interactions of PT cubosomes with lipid layers, especially DMPS-based monolayer were stronger compared with those of GMO-based nanoparticles. The kinetics of incorporation of cubosomal material into the lipid layer was influenced by the extent of hydration of the polar headgroups of the lipid: faster in the case of smaller, less hydrated polar groups of DMPS than for strongly hydrated uncharged choline of DPPC. The membrane disrupting effect of cubosomes increased at longer times of the lipid membrane exposure to the cubosome solution and at larger carrier concentrations. Langmuir monolayer observations correspond well to results of studies of HeLa cells exposed to cubosomes. The larger toxicity of PT cubosomes was confirmed by MTS. Their ability to disrupt lipid membranes was imaged by confocal microscopy. On the other hand, PT cubosomes easily penetrated cellular membranes and released cargo into various cellular compartments more effectively than GMO-based nanocarriers. Therefore, at low concentrations, they may be further investigated as a promising drug delivery tool.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorGodlewska, Marlena
dc.contributor.authorBilewicz, Renata
dc.contributor.authorNazaruk, Ewa
dc.contributor.authorMatyszewska, Dorota
dc.contributor.authorJabłonowska, Elżbieta
dc.contributor.authorGaweł, Damian
dc.date.accessioned2024-01-25T05:11:59Z
dc.date.available2024-01-25T05:11:59Z
dc.date.issued2021
dc.description.financePublikacja bezkosztowa
dc.description.number1
dc.description.volume1865
dc.identifier.doi10.1016/J.BBAGEN.2020.129738
dc.identifier.issn0304-4165
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/111317
dc.identifier.weblinkhttps://api.elsevier.com/content/article/PII:S030441652030249X?httpAccept=text/xml
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofBiochimica et Biophysica Acta - General Subjects
dc.relation.pages129738
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enConfocal microscopy
dc.subject.enCubosomes
dc.subject.enHeLa
dc.subject.enLangmuir monolayer
dc.subject.enModel lipid membrane
dc.subject.enMTS
dc.titleLipid membranes exposed to dispersions of phytantriol and monoolein cubosomes: Langmuir monolayer and HeLa cell membrane studies
dc.typeJournalArticle
dspace.entity.typePublication