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Dostęp zamkniętyRNA polymerase II-binding aptamers in human ACRO1 satellites disrupt transcription in cis
| dc.abstract.en | Transcription elongation is a highly regulated process affected by many proteins, RNAs and the underlying DNA. Here we show that the nascent RNA can interfere with transcription in human cells, extending our previous findings from bacteria and yeast. We identified a variety of Pol II-binding aptamers (RAPs), prominent in repeat elements such as ACRO1 satellites, LINE1 retrotransposons and CA simple repeats, and also in several protein-coding genes. ACRO1 repeat, when translated in silico, exhibits ~50% identity with the Pol II CTD sequence. Taken together with the observation that proteins in general tend to interact with RNAs similar to their cognate mRNAs, this suggests a mechanism for RAP binding. Using a reporter construct, we show that ACRO1 potently inhibits Pol II elongation in cis. We propose a novel mode of transcriptional regulation in humans, in which the nascent RNA binds Pol II to silence its own expression. © 2020, © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. |
| dc.affiliation | Uniwersytet Warszawski |
| dc.contributor.author | Pelchrzim, Fredrike von |
| dc.contributor.author | Boots, Jennifer |
| dc.contributor.author | Schroeder, Renée |
| dc.contributor.author | Zagrovic, Bojan |
| dc.contributor.author | Chen, Doris |
| dc.contributor.author | Zimmermann, Bob |
| dc.contributor.author | Żywicki, Marek |
| dc.contributor.author | Weiss, Adam |
| dc.contributor.author | Matylla-Kulińska, Katarzyna |
| dc.contributor.author | Radtke, Maximilian |
| dc.contributor.author | Friesacher, Theres |
| dc.date.accessioned | 2024-01-25T19:27:14Z |
| dc.date.available | 2024-01-25T19:27:14Z |
| dc.date.issued | 2020 |
| dc.description.finance | Publikacja bezkosztowa |
| dc.description.volume | 11 |
| dc.identifier.doi | 10.1080/21541264.2020.1790990 |
| dc.identifier.issn | 2154-1264 |
| dc.identifier.uri | https://repozytorium.uw.edu.pl//handle/item/118651 |
| dc.identifier.weblink | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088017926&doi=10.1080%2f21541264.2020.1790990&partnerID=40&md5=8ec186f8297f04dac465bbd41ecf6b47 |
| dc.language | eng |
| dc.pbn.affiliation | biological sciences |
| dc.relation.ispartof | Transcription |
| dc.relation.pages | 1-13 |
| dc.rights | ClosedAccess |
| dc.sciencecloud | nosend |
| dc.subject.en | regulatory RNAs |
| dc.subject.en | repeats |
| dc.subject.en | RNA aptamers |
| dc.subject.en | RNA polymerase II |
| dc.subject.en | silencing |
| dc.subject.en | Transcription |
| dc.subject.en | acrosin |
| dc.subject.en | aptamer |
| dc.subject.en | repetitive DNA |
| dc.subject.en | Article |
| dc.subject.en | chromatin immunoprecipitation |
| dc.subject.en | chromosome satellite |
| dc.subject.en | controlled study |
| dc.subject.en | flow cytometry |
| dc.subject.en | fluorescence microscopy |
| dc.subject.en | fractionation |
| dc.subject.en | gene expression |
| dc.subject.en | gene library |
| dc.subject.en | gene sequence |
| dc.subject.en | genetic transcription |
| dc.subject.en | genetic transfection |
| dc.subject.en | HeLa cell line |
| dc.subject.en | high throughput screening |
| dc.subject.en | human |
| dc.subject.en | human cell |
| dc.subject.en | hybridization |
| dc.subject.en | immunoprecipitation |
| dc.subject.en | Northern blotting |
| dc.subject.en | nucleotide sequence |
| dc.subject.en | protein binding |
| dc.subject.en | real time polymerase chain reaction |
| dc.subject.en | reverse transcription polymerase chain reaction |
| dc.subject.en | sequence alignment |
| dc.subject.en | systematic evolution of ligands by exponential enrichment aptamer technique |
| dc.subject.en | transcription regulation |
| dc.subject.en | uterine cervix adenocarcinoma |
| dc.title | RNA polymerase II-binding aptamers in human ACRO1 satellites disrupt transcription in cis |
| dc.type | JournalArticle |
| dspace.entity.type | Publication |