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A benzimidazole - based ruthenium (IV) complex inhibits Pseudomonas aeruginosa biofilm formation by interacting with siderophores and the cell envelope, and inducing oxidative stress

Autor
Czerwonka, Grzegorz
Gmiter, Dawid
Guzy, Anna
Rogala, Patrycja
Jabłońska-Wawrzycka, Agnieszka
Borkowski, Andrzej
Cłapa, Tomasz
Narożna, Dorota
Kowalczyk, Paweł
Syczewski, Marcin
Data publikacji
2019
Abstrakt (EN)

Pseudomonas aeruginosa biofilm-associated infections are a serious medical problem, and new compounds and therapies acting through novel mechanisms are much needed. Herein, the authors report a ruthenium(IV) complex that reduces P. aeruginosa PAO1 biofilm formation by 84%, and alters biofilm morphology and the living-to-dead cell ratio at 1 mM concentration. Including the compound in the culture medium altered the pigments secreted by PAO1, and fluorescence spectra revealed a decrease in pyoverdine. Scanning electron microscopy showed that the ruthenium complex did not penetrate the bacterial cell wall, but accumulated on external cell structures. Fluorescence quenching experiments indicated strong binding of the ruthenium complex to both plasmid DNA and bovine serum albumin. Formamidopyrimidine DNA N-glycosylase (Fpg) protein digestion of plasmid DNA isolated after ruthenium(IV) complex treatment revealed the generation of oxidative stress, which was further proved by the observed upregulation of catalase and superoxide dismutase gene expression.

Słowa kluczowe EN
Ruthenium complex
biofilm
benzimidazole
Pseudomonas aeruginosa PAO1
fluorescence quenching
cell surface hydrophobicity
Dyscyplina PBN
nauki chemiczne
Czasopismo
Biofouling
Tom
3
Zeszyt
1
Strony od-do
59-74
ISSN
0892-7014
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