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Label-Free Mass Spectrometry-Based Proteomic Analysis in Lamb Tissues after Fish Oil, Carnosic Acid, and Inorganic Selenium Supplementation

Autor
Gawor, Andrzej
Ruszczyńska, Anna
Konopka, Anna
Wryk, Grzegorz
Czauderna, Marian
Bulska, Ewa
Data publikacji
2022
Abstrakt (EN)

Selenium is an essential nutrient, building twenty five identified selenoproteins in humans known to perform several important biological functions. The small amount of selenium in the earth’s crust in certain regions along with the risk of deficiency in organisms have resulted in increasingly popular dietary supplementation in animals, implemented via, e.g., inorganic selenium compounds. Even though selenium is included in selenoproteins in the form of selenocysteine, the dietary effect of selenium may result in the expression of other proteins or genes. Very little is known about the expression effects modulated by selenium. The present study aimed to examine the significance of protein expression in lamb tissues obtained after dietary supplementation with selenium (sodium selenate) and two other feed additives, fish oil and carnosic acid. Label-free mass spectrometry-based proteomic analysis was successfully applied to examine the animal tissues. Protein-protein interaction network analysis of forty differently-expressed proteins following inorganic selenium supplementation indicated two significant clusters which are involved in cell adhesion, heart development, actin filament-based movement, plasma membrane repair, and establishment of organelle localization.

Słowa kluczowe EN
selenium
supplementation
label-free mass spectrometry
proteomics
protein-protein interactio
Dyscyplina PBN
nauki chemiczne
Czasopismo
Animals
Tom
12
Zeszyt
11
Strony od-do
1428
Data udostępnienia w otwartym dostępie
2022-05-31
Licencja otwartego dostępu
Uznanie autorstwa